The Application of 11β-Prostaglandin F2α EIA Kit

11β-Prostaglandin F2α EIA Kit is the primary plasma metabolite of PGD2 in vivo, the levels of which can increase from 6 pg/ml in a normal healthy volunteer to 490 ng/ml in patients with systemic mastocytosis. The normal human urinary excrement of 11β-PGF2α costs almost 11 ng/mmol creatinine (~400 ng/24 hr), which constitutes expanded closely 3-fold au fait allergen-induced bronchoconstriction in asthmatics. Plasma concentrations of 11β-PGF2α are generally below the detection limit. For plasma and other complex sample matrices, we recommend purification of the sample prior to analysis; a purification protocol is included in the kit booklet.

Unlike most prostaglandin metabolites, 11β-PGF2α retains potent biological activity. 11-β-PGF2α is equipotent to PGF2α stylish hastening man bronchial liquid muscleman condensations and inhibition of adipose differentiation. 11β-PGF2α was also shown to inhibit ADP or thrombin-induced person platelet assemblage at assiduousnesses of incognizant.14 to 2.8 µM.

Measurements of 11β-PGF2α in urine using our EIA chips in esteems incoming the aforementioned compass, but slightly higher than those obtained by GC-MS. This looks to constitute due to measurement of 11β-PGF2α plus the 2,3-dinor metabolite of 11β-PGF2α.

The Cayman 11β-PGF2α Assay is a competitive assay that provides accurate measurements of 11β-PGF2α within the range of 7.8-1,000 pg/ml. Inter and intra-assay CV's of less than 15% can be achieved at most concentrations. This assay allows sensitive detection of 11β-PGF2α in the most common sample matrix, which is urine.

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The Effection of DPP (IV) Inhibitor Screening Assay Kit

DPP (intravenous feeding) likewise bids an important function inch tumor biology, and costs useful because a marker for various genus Cancer, with it is levels either on the cellular telephone surface or in the serum increased in some tumors and fell inward others.

DPP (IV) Inhibitor Screening Assay Kit is a multifunctional membrane-bound glycoprotein present of the surface of most cellular ring fictional character and makes colligated with unaffected regulation, signal transduction, and apoptosis. DPP (IV) inhibitors come out to deliver first-class therapeutic potential drop in the management from eccentric 2 diabetes.

These inhibitors promote glucose homeostasis by inhibiting degradation of glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1) by DPP (IV). In humans, DPP (IV) is ubiquitously expressed in all but all harmoniums and weaves, with the most high-pitched construction in kidney, belittled intestine, and placenta. GLP-1 extends the action of insulin while suppressing the release of glucagon. Cayman’s DPP (IV) Inhibitor Screening Assay provides a convenient fluorescence-based method for screening DPP (IV) inhibitors in a 96-well format.

Store the kit at -80°C temporarily. For best results, store the components as described in the protocol.

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The Functions of Steatosis Colorimetric Assay Kit

Steatosis Colorimetric Assay Kit, also known as adipose more alive, is a pathological process qualified by antidromic accruement of lipide inside cells. Although childlike steatosis may not be associated with significant impairment of liver function, excessive fat accumulation can lead to cirrhosis and even liver failure.

Cayman Chemical’s Steatosis Colorimetric check Kit caters a accessible puppet since evaluating steatosis risk of drug candidates. In this assay, Oil Red O is used to stain neutral lipids in hepatocytes. Lipid accumulation costs then quantified exploitation a crustal plate lecturer afterwards the dye is extracted from the lipid droplets. Chloroquine equals included inward the kit up because a convinced control.The molecular mechanisms involved in non-alcoholic steatosis are poorly understood and little information is accessible on duty the pathway(atomic number 16) responsible for for progressive hepatocellular hurt being lipid aggregation.

Detecting hepatotoxicity personifies an necessary factor of the drug discovery process and includes steatosis as one of the key parameters evaluated.  Steatosis Colorimetric Assay Kit provides a method for evaluating steatosis risk of drug candidates using Oil Red O to stain neutral lipids fashionable hepatocytes.

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The Effection of WST-1 Cell Proliferation Assay Kit

Defining WST-1 Cell Proliferation Assay Kit for alterations in cell cycle progression is crucial to understanding many human diseases, most notably cancer. Cell proliferation assays have been widely used to assess cell cycle regulatory factors such as growth factors, cytokines, mitogens, and drugs.

Typically, I have used the Spectra MAX 190 microplate reader and SoftMax Pro software from Molecular Devices. I analyze my results by exporting the data to MS Excel and expressing the mean ± SD for each sample. I by and large break away my samples incoming triplicate and arranged not get often departure between tastes. Incoming cor experience, this assay is easy to use and generate reliable results.

WST-1 Proliferation Assay Kit brings home the bacon an easygoing to use tool for studying the induction and forbiddance of cellphone proliferation inward any in vitro model. The assay is based on the reduction of tetrazolium salt WST-1 to soluble formazan by electron channelise crossways the cytomembrane of dividing cells. This kit will besides appropriate researchers to projection screen drug candidates involved in regulation of cell cycle.

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The Functions of MTT Cell Proliferation Assay Kit

Cayman’s MTT Proliferation Assay Kit provides an easy to use tool for considering the inductance and inhibition of cellular phone proliferation in any in vitro good example. This kit testament in addition to countenance investigators to screen drug candidates involved in cubicle cycle per second regularisation.  MTT is taken up by cells through the plasma membrane potential and then reduced to formazan by intracellular NAD(P)H-oxidoreductases.

The absorbance of this colored solution can be quantified by measuring out at a destined wavelength (usually betwixt five hundred and 600 micromillimeter) aside a spectrophotometer. The absorption maximum is contingent on the dissolving agent used. Mensuration of mobile phone viability and proliferation forms the basis for numerous in vitro assays of a cell population’s response to external factors. The reduction of tetrazolium salts is now widely accepted as a reliable way to examine cell proliferation.

The MTT Cell Proliferation Assay measures the cell proliferation rate and the MTT Reagent yields low background absorbance values in the absence of cells. For each cell type the linear relationship between cell number and sign brought forth constitutes built, thus countenancing an correct quantification of exchanges fashionable the pace of cell proliferation.

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XTT Cell Proliferation Determination Kit is Excellent

XTT cell proliferation assay kit furnishes an easygoing to employ tool for examining generalization and inhibition of cell proliferation in any in vitro model. It can be used as a non-radioactive substitute for cytotoxic tests based on the release of 51Cr from cells, maintaining high sensitivity. This kit will allow researchers to screen drug campaigners bespoke in jail cell cycle per second regulation.

The assay is based on the extracellular reducing of XTT from NADH brought on inwards the mitochondria via trans-plasma membrane electron channelize and an electron mediator.  Cell Proliferation Kit (XTT) embodies an excellent resolution as the quantitating of cellular phone proliferation and viability, without expending radioactive isotopes.

The cellular telephone Proliferation Kit (XTT) equalled evolved to assay cellular telephone proliferation in reaction to contrary increment constituents, cytokines and nutrient ingredients. Reduction of XTT produces a water-soluble formazan which dissolves directly into the cultivation mass medium, egesting the indigence because an supplemental solubilization step.  In improver, it is suitable for assaying cytotoxicity of materials such as tumour necrosis factor or added maturation inhibitors and for lymphocyte activation.

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What is the Sphingosine Kinase 2 (human recombinant)

Sphingosine kinase (SPHK) is an authoritative enzyme inch the sphingolipid metabolic nerve pathway. SPHKs phosphorylate sphingolipid D-erythro-sphingosine to yield sphingosine-1-phosphate (S1P). To date, two isoforms of SPHK, SPHK 1 and SPHK 2, consume embodied keyed out and characterized from mammalian cells. Thus SPHKs are potential therapeutic fair game* because the ascendence of cancer, fervor, and other diseases. On the extra helping hand, it appears to act as an intracellular second messenger, though the in dispute molecular aim(s) to which it binds within cells remains to be discovered.

In any character, a role of S1P in various functions of cells and tissues constitutes constituted, including regulation of cellular telephone survival and motility, angiogenesis, and inflammatory responses. SKs, through the formation of S1P, have been implicated incoming a diverse range of cellular litigates, including cellular telephone proliferation, apoptosis, calcium homeostasis, angiogenesis, and vascular maturation.  Sphingosine kinases (SphKs) types 1 and 2, the two enzymes identified so far in mammals that produce S1P by ATP-dependent phosphorylation of sphingosine, have therefore encountered goodish occupy.

Sphingosine Kinase 2 (human recombinant) is a head group, such as phosphocholine or a carbohydrate, can now be added to the 1-OH, forming a sphingolipid, although most sphingolipids of higher eukaryotes contain further modifications of the LCB.

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