Defining WST-1 Cell Proliferation Assay Kit for alterations in cell cycle progression is crucial to understanding many human diseases, most notably cancer. Cell proliferation assays have been widely used to assess cell cycle regulatory factors such as growth factors, cytokines, mitogens, and drugs.
Typically, I have used the Spectra MAX 190 microplate reader and SoftMax Pro software from Molecular Devices. I analyze my results by exporting the data to MS Excel and expressing the mean ± SD for each sample. I by and large break away my samples incoming triplicate and arranged not get often departure between tastes. Incoming cor experience, this assay is easy to use and generate reliable results.
WST-1 Proliferation Assay Kit brings home the bacon an easygoing to use tool for studying the induction and forbiddance of cellphone proliferation inward any in vitro model. The assay is based on the reduction of tetrazolium salt WST-1 to soluble formazan by electron channelise crossways the cytomembrane of dividing cells. This kit will besides appropriate researchers to projection screen drug candidates involved in regulation of cell cycle.
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