Malachite Green Phosphate seek Kit leaves a fasting, reproducible, colorimetric method for measuring inorganic free phosphate in aqueous solutions. The assay method is based on duty the geological formation of a complex between malachite green molybdate and free orthophosphate that absorbs at 620-640 nm.
Applications programme for this check let in quantification of phosphorylation and phosphate release from protein phosphatase substrates. The non-radioactive colorimetric assay kits have been optimized to offer superior sensitivity and prolonged shelf life. This assay embodies a tested and eligible substance of detecting and quantifying nominal quantities of inorganic disembarrass phosphate and is amenable to high-throughput screening applications. The assay is formatted to a 96-well format, but fired easily follow restrained for habit in 384-well or cuvette-based assays.
High-throughput phosphate assay using malachite green method at 620nm. Detection limit: 0.02 M . Shelf life: 12 months. Shipping: ambient temp; storage: 2-8C .
The Malachite Green Phosphate Assay Kit is based on quantification of the green complex formed between Malachite Green, molybdate and free orthophosphate. The rapid color formation from the reaction can be conveniently measured on a spectrophotometer (600 - 660 nm) or on a plate reader.
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