What is NF-κB (human p50/p65) Combo Transcription Factor Assay Kit?

Description of NF-κB (human p50/p65) Combo Transcription Factor Assay Kit
The NF-kappaB/Rel family of transcription factors is comprised of several structurally-related proteins that form homodimers and heterodimers and include p50/p105, p52/p100, RelA (p65), and c-Rel/NF-kappaB. Acting as dimers, these transcription factors bind to kappaB sites on DNA, thereby regulating expression of target genes. The most common Rel/NF-kappaB dimer in mammals contains p50-RelA (p50/p65) heterodimers and is an attractive target for potential therapeutics in human inflammation and certain other diseases. Cayman's NF-kappaB (human p50/p65) Combo Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts. A specific double stranded DNA (dsDNA) sequence containing the NF-kappaB response element is immobilized onto the wells of a 96-well plate. NF-kappaB contained in a nuclear extract, binds specifically to the NF-kappaB response element. NF-kappaB p50 and p65 are detected by addition of specific primary antibodies directed against either NF-kappaB p50 or p65. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm.
NF-κB (human p50/p65) Combo Transcription Factor Assay Kit is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts. A specific double stranded DNA (dsDNA) sequence containing the NF-κB response element is immobilized onto the wells of a 96-well plate. NF-κB contained in a nuclear extract, binds specifically to the NF-κB response element. NF-κB p50 and p65 are detected by addition of specific primary antibodies directed against either NF-κB p50 or p65. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm.
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Specifications of ACETONE 24-DINITROPHENYLHYDRAZONE

ACETONE 24-DINITROPHENYLHYDRAZONE
CAS:1567-89-1
Molecular Formula:C9H10N4O4
Formula Weight:238.2
Description:
Specification:1ml
Melting point:126-128 °C(lit.)
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What is Sphingosine Kinase 2 (human recombinant) used for?

Formulation:
10 µg in 25 mM Tris-HCl, pH 8.0, 100 mM sodium chloride, 0.05% Tween 20, 50% glycerol, and 3mM DTT
Description of Sphingosine Kinase 2 (human recombinant)
Sphingosine kinase (SPHK) is an important enzyme in the sphingolipid metabolic pathway. SPHKs phosphorylate sphingolipid D-erythro-sphingosine to yield sphingosine-1-phosphate (S1P). To date, two isoforms of SPHK, SPHK 1 and SPHK 2, have been identified and characterized from mammalian cells. SKs, through the formation of S1P, have been implicated in a diverse range of cellular processes, including cell proliferation, apoptosis, calcium homeostasis, angiogenesis, and vascular maturation. Thus SPHKs are potential therapeutic targets for the control of cancer, inflammation, and other diseases.
Purity:>80% by SDS-PAGE
Human Sphingosine kinase 2
(GenBank Accession No. AF245447), full
length with N-terminal His tag, MW=69.5 kDa,
expressed in a Baculovirus infected Sf9 cell
expression system.
Application
Useful for the study of enzyme kinetics,
screening inhibitors, and selectivity
profiling.
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What is Trisodium phosphate?

Trisodium phosphate (TSP, E339) is a cleaning agent, food additive, stain remover and degreaser. It is a white, granular or crystalline solid, highly soluble in water producing an alkaline solution. The item of commerce is often partially hydrated and may range from anhydrous trisodium phosphate, Na3PO4, to the dodecahydrate, Na3PO4·12H2O. Most often found in white powder form, it can also be called trisodium orthophosphate or just plain sodium phosphate. Trisodium phosphate was at one time extensively used in formulations for a wide variety of consumer grade soaps and detergents, but ecological problems have largely ended that practice, at least in the western world. Substitutes are not as effective, but the raw chemical can be bought in bulk to add to underpowered detergents.
Uses
Cleaning
The major use for trisodium phosphate is in cleaning agents. The pH of a 1% solution is 12, and the solution is sufficiently alkaline to saponify grease and oils. In combination with surfactants, TSP is an excellent agent for cleaning everything from laundry to concrete driveways. This versatility and low manufacturing price, made TSP the preferred basis for a plethora of cleaning products sold in the mid-20th century. TSP is still sold, and used, as a cleaning agent, but during the late 1960s in the United States,[citation needed] government regulators in seventeen states determined that overuse led to a series of ecological problems.
By the end of the 20th century, many products that formerly contained TSP were manufactured with TSP Substitutes, which consist mainly of sodium carbonate along with various admixtures of nonionic surfactants and a limited percentage of sodium phosphates.
Trisodium phosphate is commonly used after cleaning with mineral spirits in order to remove hydrocarbon residues. TSP may be used with household chlorine bleach in the same solution without hazardous reactions. This mixture is particularly good for removing mildew.
Although it is still the active ingredient in some toilet bowl cleaning tablets, TSP is generally not good for cleaning bathrooms, because it can stain metal and can damage grout.
Flux
In the United States, trisodium phosphate is an approved flux for use in hard soldering joints in medical grade copper plumbing. The flux is applied as a concentrated water solution and dissolves copper oxides at the temperature used in copper brazing. Residues are fully water soluble and can be rinsed out of plumbing before it is put in service.
Trisodium phosphate is used as an ingredient in fluxes designed to deoxygenate nonferrous metals for casting.
TSP can be used in ceramic production to lower the flow point of glazes.
Painting enhancement
TSP is still in common use for the cleaning, degreasing and deglossing of walls prior to painting. Trisodium phosphate breaks the gloss of oil based paints and opens the pores of latex based paint providing a surface better suited for the adhesion of the subsequent layer of paint.
Food additive
Trisodium phosphate is approved as a food additive in the EU.
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Specifications o Antimony potassium tartrate

Antimony potassium tartrate
SYNONYMS: Tartar Emetic; Tartaric acid, antimony potassium salt, trihydrate; Potassium antimony(III) oxide tartrate hemihydrate;  Dipotassium bis[mu-[tartrato(4-)-O1,O2:O3, O4]] diantimonate(2-); Bis[.mu.-[tartrato(4-)-O1,O2:O3,O4]]diantimonato(2-) de dipotasio (Spanish);
SOLUBILITY IN WATER: moderately soluble (soluble in glycerine, insoluble in EtOH)
Antimony potassium tartrate is used as a mordant or fixing agent in the leather and textile dying as well as an analytical reagent and a flux additive for electoplating. It is used in making insecticides or pesticides. It was used as a parasiticide or as an emetic and expectorant.
Antimony Potassium Tartrate is a colourless odourless poisonous crystalline salt used as a mordant for textiles and leather, as an insecticide, and as an anthelmintic. Formula: K(SbO)C4H4O6 Also called tartar emetic.
Antimony potassium tartrate: a compound used as an expectorant and in the treatment of schistosomiasis japonicum, although the drug is extremely toxic and must be administered slowly intravenously; common toxic manifestations are phlebitis, tachycardia, and hypotension; sudden deaths have been reported, chiefly from circulatory collapse.
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Where to find Cytochrome b5 (human)?

Cytochrome b5 (human) (gene name CYB5), is a human microsomal cytochrome b5.
Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. It has two isoforms produced by alternative splicing. Isoform 1 is bound to the cytoplasmic side of the endoplasmic reticulum. It has a C-terminal transmembrane alpha-helix. Isoform 2 was found in cytoplasm. Defects in CYB5A are the cause of type IV hereditary methemoglobinemia.
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What is Sodium caseinate?

Sodium caseinate is a tasteless, odorless, water-soluble, white powder; used in medicine, foods, emulsification, and stabilization; formed by dissolving casein in sodium hydroxide and then evaporating. Also known as casein sodium; nutrose.
Sodium caseinate is used as source of protein & sodium in health foods, baby foods, bacteriological media, milk whitener, nutraceutical preparations, meat & food industries as an extender in meat products. Sodium Caseinate Powder is a spray dried Sodium Caseinate, free flowing, slightly hygroscopic white to off white colored powder obtained from high grade purified casein. Sodium Caseinate (Spray Dried) is also used as a additive in weaving products as a binder.
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Where to buy Cytochrome P450 1B1 (human) Yeast Reductase?

Synonyms CYP1B1 YR
Stability 2 years
Storage -80°C
Cytochrome P450 1B1 (human) Yeast Reductase is found mainly in extrahepatic tissues and is overexpressed in a smorgasbord of human tumors. In contrast, V79 cells stably expressing human P450 1B1 generated exclusively DB[a,l]PDE-DNA adducts. Differences in the total level of DNA binding were also observed.
Metabolic activation of 17β-estradiol (E2) to 4-hydroxy E2 by CYP1B1 has been postulated to be an important factor in mammary carcinogenesis. The inhibition of recombinant human CYP1B1 by 2,2′,4,6′-tetramethoxystilbene (TMS) was investigated using either the Escherichia coli membranes of recombinant human CYP1B1 coexpressed with human NADPH-P450 reductase or using purified enzyme. 2,2′,4,6′-TMS showed potent and selective inhibition of ethoxyresorufin O-deethylation (EROD) activity of CYP1B1 with IC50 values of 2 nM. 2,2′,4,6′-TMS exhibited 175-fold selectivity for CYP1B1 over CYP1A1 (IC50, 350 nM) and 85-fold selectivity for CYP1B1 over CYP1A2 (IC50, 170 nM).
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What is Sphingosine Kinase 2 (human recombinant)?

Description of Sphingosine Kinase 2 (human recombinant)
Source: human recombinant N-terminal His-tagged protein from Sf9 cells · Mr: 69.5 kDa · Sphingosine kinase (SPHK) is an important enzyme in the sphingolipid metabolic pathway. SPHKs phosphorylate sphingolipid D-erythro-sphingosine to yield sphingosine-1-phosphate (S1P). To date, two isoforms of SPHK, SPHK 1 and SPHK 2, have been identified and characterized from mammalian cells. SKs, through the formation of S1P, have been implicated in a diverse range of cellular processes, including cell proliferation, apoptosis, calcium homeostasis, angiogenesis, and vascular maturation. Thus SPHKs are potential therapeutic targets for the control of cancer, inflammation, and other diseases.
Sphingosine kinase 2, full length with N-terminal His tag, MW=74 kDa, expressed in a Baculovirus infected Sf9 cell expression system Source: Baculovirus infected Sf9 cells Fomulated in: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT. Stability: >6 months at 80°C Purity: >80% by SDS-PAGE. Specific Activity: 20 U/ug. Sphingosine kinase activity was determined using D-erythrosphingosine and ATP as substrates. A unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P/min.
Applications   
useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
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Specifications of Hexachloroiridic acid hexahydrate

Hexachloroiridic acid hexahydrate
CAS:16941-92-7
Molecular Formula:Cl6H2Ir
Formula Weight:515.05
Description:
Melting point:65℃
Appearance:black crystallization.
Dissolubility:Easily deliquescent in wet air,soluble in water , hydrochloric acid and alcohol.
Use: For manufacturing coating electrode ,is an important catalyst of chemical industry and material of Iridium reagent .
Storage: Kept with sealing tight in normal temperature.
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Specifications of Tridecanoin (Caprin)C10 to 0

Tridecanoin (Caprin)C10 to 0
CAS:621-71-6
Molecular Formula:C33H62O6
Formula Weight:554.84
Description:
Specification:10mg
Melting point:31-32 °C
Boiling point:254 °C / 5mmHg
Storage:-20°C
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How to buy Akt (Isoforms 1, 2, 3) Inhibitory Peptide Set?

Akt (Isoforms 1, 2, 3) Inhibitory Peptide Set  is a protein kinase that plays a central role in inhibiting apoptosis through promoting cell survival. Activated Akt functions by phosphorylating downstream targets in survival signaling pathways. TCL1 (a proto-oncogene underlying human T-cell prolymphocytic leukemia) interacts with Akt through an N-terminal pleckstrin homology (PH) domain and functions as an Akt kinase co-activator. This inhibitory peptide interacts with Akt.1.
Akt is a protein kinase that plays a central role in inhibiting apoptosis through promoting cell survival. Activated Akt functions by phosphorylating downstream targets in survival signaling pathways. TCL1 (a proto-oncogene underlying human T-cell prolymphocytic leukemia) interacts with Akt through an N-terminal pleckstrin homology (PH) domain and functions as an Akt kinase co-activator. This inhibitory peptide interacts with Akt.1 The peptide binds to the PH domain of Akt and inhibits Akt1, Akt2, and Akt3 kinase activity. The Akt (Isoforms 1, 2, 3) inhibitory peptide also contains a protein transduction (PTD) sequence (DRQIKIWFQNRRMKWKK) derived from antennapedia which renders the peptide cell permeable.2 The control peptide consists of only the PTD sequence.
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Properties of Urea

Urea or carbamide is an organic compound with the chemical formula CO(NH2)2. The molecule has two —NH2 groups joined by a carbonyl (C=O) functional group.
Urea serves an important role in the metabolism of nitrogen-containing compounds by animals and is the main nitrogen-containing substance in the urine of mammals. It is solid, colourless, and odorless.
It is highly soluble in water and non-toxic. Dissolved in water, it is neither acidic nor alkaline. The body uses it in many processes, the most notable one being nitrogen excretion. Urea is widely used in fertilizers as a convenient source of nitrogen. Urea is also an important raw material for the chemical industry. The synthesis of this organic compound by Friedrich Wöhler in 1828 from an inorganic precursor was an important milestone in the development of organic chemistry, as it showed for the first time that a molecule found in living organisms could be synthesized in the lab without biological starting materials.
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What is Cavβ4 Calcium Channel Monoclonal Antibody (Clone S10-7)?

Formulation: 100 µg of protein G-purified IgG in 100 µl PBS, pH 7.4, containing 50% glycerol and 0.09% sodium azide
Application of Cavβ4 Calcium Channel Monoclonal Antibody (Clone S10-7)
WB, IHC, ICC, and IF · Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces.
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Uses of Iridium trichloride

Iridium trichloride is the inorganic compound with the formula IrCl3.
Uses
Hydrated Iridium trichloride is used in the laboratory for the preparation of other iridium compounds such as Vaska's complex, trans-[IrCl(CO)(PPh3)2]. Alkene complexes such as the dimeric {Ir(COD)Cl}2 and {Ir(cycloctene)2Cl}2 can also be prepared by heating the trichloride with the appropriate alkene in water/alcohol mixtures. Industrially, most iridium complexes are generated from H2IrCl6 or (NH4)2IrCl6 as these salts are the most common commercial forms of iridium chlorides, being directly obtained in the purification of iridium.
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What is Hexadecane?

Hexadecane (also called cetane) is an alkane hydrocarbon with the chemical formula C16H34. Hexadecane consists of a chain of 16 carbon atoms, with three hydrogen atoms bonded to the two end carbon atoms, and two hydrogens bonded to each of the 14 other carbon atoms.
Cetane is often used as a short-hand for cetane number, a measure of the detonation of diesel fuel. Cetane ignites very easily under compression; for this reason, it is assigned a cetane number of 100, and serves as a reference for other fuel mixtures.
Used:Used to produce various maleic anhydride copolymers.
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What is Cytochrome b5 (human)?

Cytochrome b5 (human) is a human microsomal cytochrome b5.
Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. It has two isoforms produced by alternative splicing. Isoform 1 is bound to the cytoplasmic side of the endoplasmic reticulum. It has a C-terminal transmembrane alpha-helix. Isoform 2 was found in cytoplasm. Defects in CYB5A are the cause of type IV hereditary methemoglobinemia.
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Applications of Ruthenium on carbon

Applications of Ruthenium on carbon
Ruthenium Carbon catalyst has been used in the hydrogenation of glucose to sorbitol successfully.
Ruthenium on carbon is a suitable one for hydrogenation of substituted anilines to cyclohexylamines, carboxylic acids to alcohols, allyl and heterocyclic aromatic compounds, Fischer-Tropsch synthesis, etc.
Appearance:black powder
Melting point:2310 °C(lit.)
Boiling point:3900 °C(lit.)
Storage: Kept with sealing tight.
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The Application of Hydrazine Acetate

Hydrazine acetate is C2H8N2O2. Hydrazine marketed for rocket propellant contains a minimum of 97 per cent N2H4, the other constituent being primarily water. Hydrazine is a clear, water-white, hygroscopic liquid. The solid is white. Hydrazine a toxic, flammable caustic liquid and a strong reducing agent. Its odour is similar that of ammonia, though less strong. Increased incidences of lung, nasal cavity, and liver tumors have been observed in rodents exposed to hydrazine. EPA has classified hydrazine as a Group B2, probable human carcinogen. It is slightly soluble in ammonia and methyl-amine. It is soluble in water, methanol, ethanol, UDMH, and ethylenediamine.

Hydrazine is a highly toxic and unstable chemical that shares properties similar to that of Ammonia. It is a chemical compound made up of nitrogen and hydrogen. Even though it is very poisonous Hydrazine has many effective applications that are becoming of greater use to scientists and researchers looking for useful sources of energy for space flight and renewable energy every vehicles.

This is becoming evident with further research into the processes used to make it and its sister compound.  Hydrazine is used in agricultural chemicals (pesticides), chemical blowing agents, pharmaceutical intermediates, photography chemicals, boiler water treatment for corrosion protection, textile dyes, and as fuel for rockets and spacecraft.

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The New Information about Nitrite Colorimetric Assay Kit

Chemical Nitrate/Nitrite Assay Kit provides an accurate and convenient method for measurement of total nitrate/nitrite concentration in a simple two-step process. Photometric measurement of the absorbance due to this azo chromophore accurately finds out nitrite assiduousness. It brings considerably for the analysis of fluids such as plasma and urine, and is also available in a high sensitivity fluorometric version for the detection of low levels of nitrate and nitrite.

This kit can be used to measure nitrate and nitrite in plasma/serum, urine, tissue culture media, and tissue homogenates. However, it cannot be exploited to psychoanalyze nitrate and nitrite from an in vitro assay of nitric oxide synthase (NOS) in which excess NADPH delivers embodied bestowed. For these assays a second method (LDH method) is utilized and is available from  Chemical in a 96 well plate format. However, this method cannot be used to analyze nitrate and nitrite from an in vitro NOS assay in which excess NADPH has been added. The second way to handle the NADPH interference problem is to go ahead and use an excess of NADPH. An additional step is added to the protocol to remove the excess NADPH. This is the avenue we have taken in the development of this nitrate/nitrite assay kit.

Each kit comprises check cushion, nitrate reductase, cofactors homework, nitrate monetary standard, lactate dehydrogenase, Griess Reagent R1, Griess Reagent R2, a 96 well plate, and complete instructions.

More about:  Nitrate/Nitrite Colorimetric Assay Kit

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The New Information about Cavβ4 Calcium Channel Monoclonal Antibody

Cavβ4 Calcium Channel Monoclonal Antibody described in this record plays an important role in calcium channel function by modulating G protein inhibition, increasing peak calcium current, controlling the a1 subunit membrane targeting and shifting the voltage dependence of activation and inactivation. Certain mutations in this gene have been associated with idiopathic generalized epilepsy (IGE) and juvenile myoclonic epilepsy (JME).

They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage-gated ion channels, ligand-gated, other gating systems, and finally those that are classified differently, having more exotic characteristics. The first are voltage-gated ion channels which open and close in response to membrane potential. These are then seperated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels, each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein.

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The New Information about Cavβ4 Calcium Channel Monoclonal Antibody

Cavβ4 Calcium Channel Monoclonal Antibody described in this record plays an important role in calcium channel function by modulating G protein inhibition, increasing peak calcium current, controlling the a1 subunit membrane targeting and shifting the voltage dependence of activation and inactivation. Certain mutations in this gene have been associated with idiopathic generalized epilepsy (IGE) and juvenile myoclonic epilepsy (JME).

They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage-gated ion channels, ligand-gated, other gating systems, and finally those that are classified differently, having more exotic characteristics. The first are voltage-gated ion channels which open and close in response to membrane potential. These are then seperated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels, each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein.

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How to Use WST-1 Cell Proliferation Assay Kit Correctly

Defining the mechanisms responsible for alterations in cell cycle progression lives determinative to empathizing several person diseases, most notably cancer. Cell proliferation assays have been widely used to assess cell cycle regulatory factors such as growth factors, cytokines, mitogens, and drugs. You do not need to remove culture media or dampen with phosphate buffer solution since this assay. It is simple, just add 10 ul of WST-1 reagent to 100 ul of media in a 96-well plate. The reduced caring compulsory with WST-1 means that there is a reduced chance of errors due to pipetting/handling.

The colorimetric assay is based on the cleavage of the tetrazolium salt WST-1 to a formazan-class dye by mitochondrial succinate-tetrazolium reductase in viable cells (1). As the cells proliferate, more WST-1 is converted to the formazan mathematical product (compute 1, below).

The quantity of formazan dye is directly related to the number of metabolically active cells, and can be quantified by measuring the absorbance at 420–480 nm (Amax 450 nm) in a multiwell plate reader.

WST-1 Proliferation Assay Kit provides an easy to use tool for studying the induction and inhibition of cell proliferation in any in vitro model. The assay is based on the reduction of tetrazolium salt WST-1 to soluble formazan by electron transport across the plasma membrane of parting cubicles. This kit will also allow detectives to concealment drug nominees called for stylish regularization of cell cycle.

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What is Steatosis Colorimetric Assay Kit Used for

Steatosis, also known as fatty more 54, equals a pathological cognitive process characterized by brachydactylic accumulation of lipid within cells. While simple steatosis may not be associated with significant impairment of liver function, excessive fat accumulation can lead to cirrhosis and regular more alive failure.  teatosis Colorimetric Assay Kit brings home the bacon a process for evaluating steatosis risk of drug candidates habituating oil color blood-red atomic number 8 to stain neutral lipids in hepatocytes.

The molecular mechanisms involved in non-alcoholic steatosis are poorly understood and little information is available on the pathway(s) responsible for progressive hepatocellular damage following lipid accumulation. Determining hepatotoxicity is an all important constituent from the drug uncovering cognitive operation and includes steatosis as one of the key parameters evaluated.

Determining hepatotoxicity is an essential element of the drug discovery cognitive operation and includes steatosis as one of the key parameters evaluated. Steatosis Colorimetric Assay Kit provides a convenient tool for evaluating steatosis risk of drug candidates. In this assay, Oil Red O is used to stain neutral lipids in hepatocytes. Lipid accumulation is then quantified using a plate reader after the dye is extracted from the lipid droplets. Chloroquine is included fashionable the kit as a positive control.

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