The NF-kappaB/Rel family of transcription factors is comprised of several structurally-related proteins that form homodimers and heterodimers and include p50/p105, p52/p100, RelA (p65), and c-Rel/NF-kappaB. Acting as dimers, these transcription factors bind to kappaB sites on DNA, thereby regulating expression of target genes. The most common Rel/NF-kappaB dimer in mammals contains p50-RelA (p50/p65) heterodimers and is an attractive target for potential therapeutics in human inflammation and certain other diseases. Cayman's NF-kappaB (human p50/p65) Combo Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts. A specific double stranded DNA (dsDNA) sequence containing the NF-kappaB response element is immobilized onto the wells of a 96-well plate. NF-kappaB contained in a nuclear extract, binds specifically to the NF-kappaB response element. NF-kappaB p50 and p65 are detected by addition of specific primary antibodies directed against either NF-kappaB p50 or p65. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm.
NF-κB (human p50/p65) Combo Transcription Factor Assay Kit is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts. A specific double stranded DNA (dsDNA) sequence containing the NF-κB response element is immobilized onto the wells of a 96-well plate. NF-κB contained in a nuclear extract, binds specifically to the NF-κB response element. NF-κB p50 and p65 are detected by addition of specific primary antibodies directed against either NF-κB p50 or p65. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm.
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