Defining the mechanisms responsible for alterations in cell cycle progression lives determinative to empathizing several person diseases, most notably cancer. Cell proliferation assays have been widely used to assess cell cycle regulatory factors such as growth factors, cytokines, mitogens, and drugs. You do not need to remove culture media or dampen with phosphate buffer solution since this assay. It is simple, just add 10 ul of WST-1 reagent to 100 ul of media in a 96-well plate. The reduced caring compulsory with WST-1 means that there is a reduced chance of errors due to pipetting/handling.
The colorimetric assay is based on the cleavage of the tetrazolium salt WST-1 to a formazan-class dye by mitochondrial succinate-tetrazolium reductase in viable cells (1). As the cells proliferate, more WST-1 is converted to the formazan mathematical product (compute 1, below).
The quantity of formazan dye is directly related to the number of metabolically active cells, and can be quantified by measuring the absorbance at 420–480 nm (Amax 450 nm) in a multiwell plate reader.
WST-1 Proliferation Assay Kit provides an easy to use tool for studying the induction and inhibition of cell proliferation in any in vitro model. The assay is based on the reduction of tetrazolium salt WST-1 to soluble formazan by electron transport across the plasma membrane of parting cubicles. This kit will also allow detectives to concealment drug nominees called for stylish regularization of cell cycle.
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