Monday, February 6, 2012

The New Information about Nitrite Colorimetric Assay Kit

Chemical Nitrate/Nitrite Assay Kit provides an accurate and convenient method for measurement of total nitrate/nitrite concentration in a simple two-step process. Photometric measurement of the absorbance due to this azo chromophore accurately finds out nitrite assiduousness. It brings considerably for the analysis of fluids such as plasma and urine, and is also available in a high sensitivity fluorometric version for the detection of low levels of nitrate and nitrite.
This kit can be used to measure nitrate and nitrite in plasma/serum, urine, tissue culture media, and tissue homogenates. However, it cannot be exploited to psychoanalyze nitrate and nitrite from an in vitro assay of nitric oxide synthase (NOS) in which excess NADPH delivers embodied bestowed. For these assays a second method (LDH method) is utilized and is available from  Chemical in a 96 well plate format. However, this method cannot be used to analyze nitrate and nitrite from an in vitro NOS assay in which excess NADPH has been added. The second way to handle the NADPH interference problem is to go ahead and use an excess of NADPH. An additional step is added to the protocol to remove the excess NADPH. This is the avenue we have taken in the development of this nitrate/nitrite assay kit.
Each kit comprises check cushion, nitrate reductase, cofactors homework, nitrate monetary standard, lactate dehydrogenase, Griess Reagent R1, Griess Reagent R2, a 96 well plate, and complete instructions.

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